Surface characteristics of pure titanium loaded graphene oxide: effect on bacteria adhesion and osteoblast structure / 华西口腔医学杂志

OBJECTIVE@#To evaluate the process characterization of graphene oxide loaded on pure titanium surface and effect on the biological properties of Staphylococcus aureus and osteoblasts.@*METHODS@#Graphene oxide at four concentrations (20, 50, 80, and 100 µg·mL⁻¹) was loaded on the pure titanium surface via electroplating, and the morphology, properties, and hydrophilic properties were measured with a field emission scanning electron microscope, micro Raman spectrometer, and contact angle tester, respectively. In addition, Staphylococcus aureus and osteoblasts were used as models and cultured with pure titanium-graphene oxide. Then, field-emission scanning electron microscopy and laser confocal microscopy were utilized to observe the changes in the amount of bacteria and osteoblast morphology and structure, respectively.@*RESULTS@#Graphene oxide at the four concentrations was successfully loaded on pure titanium surface via electroplating. It improved the hydrophilic properties of pure titanium surface, which benefitted the adhesion and growth of Staphylococcus aureus and changed the morphology and structure of the osteoblasts.@*CONCLUSIONS@#The pure titanium-graphene oxide composite has no antibacterial properties and has good biocompatibility.

Analysis of influence of sarcopenia on early postoperative function of femoral neck fracture by hip arthroplasty / 中国骨伤

<p><b>OBJECTIVE</b>To determine the association of sarcopenia with short-term postoperative function after hip replacement for femoral neck fractures.</p><p><b>METHODS</b>A prospective study of 181 consecutive patients with femoral neck fractures who underwent hip replacement from May 2014 to January 2017 were performed, including 58 males and 123 females aging from 53 to 92 years old. The general conditions were collected before surgery, skeletal muscle index(ASMI), handgrip strength were measured. Clinical outcomes were followed up including postoperative complications, time of on-site, Harris score (postoperative 2 weeks, 3, 6 months), hospitalization costs, and hospital stay. According to handgrip strength and ASMI, the patients were divided into the sarcopina group and the non-sarcopina group; according to the Harris score at the 6-month follow-up, the patients were divided into good prognosis group and poor prognosis group. Univariate analysis and binary logistic regression analysis were used to investigate whether sarcopenia was a risk factor for poor postoperative hip joint surgery.</p><p><b>RESULTS</b>All patients were followed up at 2 weeks, 3 and 6 months, postoperative early complication included wound infection in 16 cases, thrombus of lower extremity veins in 14 cases, no dislocation, prosthetic loosening and prosthesis related infections occurred. Sarcopenia was present in 82 of 181 patients(45%), Compared with non-sarcopenic patients, sarcopenic patients had a higher risk of postoperative complications, longer postoperative hospital stay, more hospital costs and lower harris scores. In Binary logistic analysis revealed that sarcopenia(=0.08), hemiarthroplasty(<0.001), diabetes(=0.016) and infection(=0.018) were important predictors of unsatisfactory postoperative function.</p><p><b>CONCLUSIONS</b>Sarcopenia is an important predictor of poor postoperative prognosis in patients with femoral neck fractures after hip replacement. The treatment for sarcopenia maybe an important way to protect patients with femoral neck fractures from poor prognosis after hip replacement.</p>

Analysis on related risk factors of hip fracture patient with sarcopenia / 中国骨伤

<p><b>OBJECTIVE</b>To explore occurrence and risk factors of sarcopenia in patients with hip facture.</p><p><b>METHODS</b>From May 2013 to January 2017, 187 patients with hip fractures were collected, including 99 males and 88 females aged from 50 to 95 years old with an average age of (77.40±10.58) years old. General conditions, appendicular skeletal muscle index (ASMI), total fat mass, bone mineral content (BMC), body mass index (BMI), grip strength, preoperative blood vitamin D (VITD), albumin, American Society of Anesthesiologists(ASA) classification and new mobility scores(NMS) were observed and analyzed. According to grip strength and ASMI, patients were divided into sarcopina group and non-sarcopina group, univariate and multivariate statistical methods were used to analyzed.</p><p><b>RESULTS</b>Ninty-nine patients(52.9%) were diagnosed as sarcopenia. Compared with non-sarcopenia group, occurrence of sarcopenia was associated with advanced age, high ASA, low total fat mass, low bone mineral content, low BMI, low albumin, and low NMS. Subsequent binary logistic regression analysis showed that advanced age (OR=1.804, =0.048), high ASA score (OR=3.052, =0.001), low fat content (OR=0.843, =0.006), low bone mineral salt(OR=0.203, =0.026) were risk factors of hip fracture patient with sarcopenia.</p><p><b>CONCLUSIONS</b>Old age, high ASA score, low fat content, low bone mineral content may be related risk factors for sarcopenia in hip fracture patients.</p>

Cloning and expression of spinach glycolate oxidase in Escherichia coli / 生物工程学报

The cDNA coding spinach glycolate oxidase (GO) was amplified by RT-PCR using the total RNA of spinach leaves as the template, and was cloned into cloning vector pMD18-T. After the DNA sequence was determined, the go gene was subcloned into E. coli expression vector pBV220, pET-22b(+), pTIG-Trx and pThioHisC. SDS-PAGE analysis revealed that the recombinant E. coli BL21 (DE3) (pTIG-Trx-GO) and E. coli BL21 (DE3) (pET-22b(+)-GO) expressed the predicted 38 kD glycolate oxidase, and the enzyme activity was also detected.